Phylum:Zygomycota >> Class: Zygomycetes >>  Order: Mucorales 
 BCRC Number NO BCRC Number!  
 Scientific Name: Blakeslea trispora

Blakeslea trispora Thaxter, Bot. Gaz. 58: 353. 1914.

Basionym:Choanephora trispora (Thaxter) Sinha, Proc. Indian Acad. Sci. Sec. B 11:167. 1940.

 Description: Colonies rapidly growing on CMA at 25°C, aerial mycelium white, abundant, densely curved and highly branched in places. Sporangiophores bearing sporangia usually arising from substrate mycelium, erect or ascending, unbranched, 1-4 mm high, hyaline, smooth walled, 13-20 µm wide. Sporangia circinately borne when young, sub-globose, (30-) 60-90 (-210) µm diameter, initially white, turning to very dark brown with age. At maturity, sporangial wall persistent, brown in colour, encrusted with fine spines, dehiscing into two segments, columella absent in smaller sporangia, when present, obovoid to pyriform, hyaline, (40-) 55-90 (-100) µm long, 30-40 µm wide, usually with a collar in the dehisced sporangium. Sporangiospores from sporangia, ellipsoid to broadly ellipsoid, reddish-brown, longitudinally striate, (10-) 12-18 µm long, (5-) 6-8 µm wide, with a group of fine, hyaline appendages at each pole, up to twice the length of the spore. Sometimes, however, the fine appendages were not easy to be observed.Sporangiophores bearing sporangiola arising from substrate mycelium or aerial hyphae, erect or ascending, 1-5 mm high, 13-20 µm wide, proximally unbranched, smooth-walled throughout, hyaline, dichotomously or subdichtomously divided distally up to 5 times, non-septate, branches usually with irregular successive constrictions, ultimate branches terminating in spherical enlargements, 22-35 µm diameter upon which the sporangiola are borne over their entire surface, each sporangiophore bearing (1-) 8-16 (-32) enlargements. Sporangiola broadly ellipsoid, attached to the spherical enlargements by a small spherical pedicel, 3-5 µm long, 2.5 µm wide, containing 3 (-4) sporangiospores, with a fragile but persistent wall, hyaline, breaking along preformed sutures at maturity into 2 segments. Sporangiospores from sporangiola ellipsoid to broadly ellipsoid, reddish- brown, longitudinally striate, 12.5-15 (-18) µm long, 6-7.5 (-8) wide, with a group of fine, hyaline appendages at each pole often equal to the length of the spore. Zygospores usually formed below the surface of the medium, 40-80 µm diameter, spherical to slightly flattened, often with a large central oil droplet at maturity, outer wall layer thin, with striation, inner wall layer thick, smooth, suspensors tongs-like and apposed, non-appendaged, arising from twisted and distorted hyphae, up to 10-20 µm diameter, often slightly unequal, heterothallic.

S11701, isolated from soil, orange garden, Yunlin County, July, 2003, collected by C.-C. Lin; S41201, isolated from soil, pear garden, Taichung County, August, 2003, collected by C.-C. Lin; N001, isolated from fallen flower of Luffa cylindrical (L.) Loem., Nantou County, May. 1996, collected by L.-L. Chang.; N002, isolated from fallen flower of Luffa cylindrical (L.) Loem. , Nantou County, May. 1996, collected by L.-L. Chang.

 Habitat: null

Brunei; China; India; Malaya; Nigeria; Sierra Leone; Taiwan; USA.


Kirk, PM. 1984; Ho, HM. & Chang, LL. 2003.


H. M. Ho

 Note: This species is characterized by the 3-4 spored sporangiola. The difference between B. monospora and B. trispora is the sporangiola which are with three to four spores in the later species.